Zapit Optostim
  • Zapit Optostim: Introduction
    • Feature List
    • FAQ
  • Installation and Setup
    • Hardware setup
    • Software setup
    • Configuring Zapit
      • General settings
      • NI DAQ settings
      • Experiment settings
      • Scanner & Camera settings
      • Laser Power Calibration
      • Blanking settings
    • Staying up to date
  • Using the GUI
    • Introduction: Using the GUI
    • Scanner Calibration
    • Sample Calibration
    • StimConfig Editor
    • Generating stimulus patterns at the CLI
    • Advanced Usage
  • Running Experiments
    • Introduction: Running Experiments
    • MATLAB
      • Basic API Usage in MATLAB
      • Running an Experiment in MATLAB
    • Python
      • Connecting Python to Zapit
      • Basic Zapit API Use in Python
    • Remote Control and Other Programming Languages
  • Developer Notes
    • Project Structure
    • Conventions
    • Hints and Tips
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  1. Installation and Setup
  2. Configuring Zapit

Experiment settings

experiment

The values in this section are used as defaults when a new stimulus configuration file is created. You can change the exact value in the stimulus configuration GUI, so here you need to only choose the most common values you expect to use. Likely you can leave all of these as they are.

  • defaultDutyCycleHz is the default duty cycle (the frequency) at which the laser will stimulate each point. A value of 40 Hz or 50 Hz is commonly used in the field.

  • defaultLaserPowerMWdefines the time-averaged power in mW at the sample surface used for stimulation. In other words, if two points are being stimulated in a single trial and requested power is 2 mW then the laser would deliver 4 mW at the sample. A power meter receiving light from just one of those points would report an average power of 2 mW.

  • offRampDownDuration_ms defines the time course over which the laser will decay from the chosen stimulation power in mW to zero mW. About 250 ms has been shown to be a good choice.

  • blankingTime_ms defines the number of ms during which the beam is switched off and the scanners move from one point to the next. The default value is suitable for faster scanners, like those from ScannerMax or CamTech. You don't need to change this now

  • blankOnsetShift_ms and blankOffsetShift_ms are used to control the relative time of the laser blanking. You should probably verify these settings, but you can not do it yet.

Once you have finished editing these, you can save the settings file.

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Last updated 9 months ago